MCP๋กœ ์—ฐ๊ฒฐ โ†’

๊ณ„์‚ฐ ์ž…๋ ฅ

๊ณต์‹

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  1. Diluent Volume

    Diluent Volume: ํฌ์„๋ฐฐ์ˆ˜ ๊ณ„์‚ฐ๊ธฐ

    Volume of diluent added = Final volume minus Initial volume

๊ด‘๊ณ 

๊ฒฐ๊ณผ

ํฌ์„๋ฐฐ์ˆ˜
10
(expressed as 1:10)
ํฌ์„ ๋ฐฐ์œจ 10-fold
์ถ”๊ฐ€ํ•  ํฌ์„์•ก(์šฉ๋งค) 9
์ตœ์ข… ๋ถ€ํ”ผ 10

ํฌ์„๋ฐฐ์ˆ˜๋ž€?

ํฌ์„๋ฐฐ์ˆ˜(DF, Dilution Factor)๋Š” ์‹œ๋ฃŒ๋ฅผ ๋ช‡ ๋ฐฐ๋กœ ํฌ์„ํ–ˆ๋Š”์ง€๋ฅผ ๋‚˜ํƒ€๋‚ด๋Š” ๊ฐ’์ž…๋‹ˆ๋‹ค. ์ตœ์ข…(์ „์ฒด) ๋ถ€ํ”ผ๋ฅผ ์ดˆ๊ธฐ ์‹œ๋ฃŒ ๋ถ€ํ”ผ๋กœ ๋‚˜๋ˆ„์–ด ๊ตฌํ•ฉ๋‹ˆ๋‹ค. ํฌ์„ํ•˜๋ฉด ๋ถ€ํ”ผ๊ฐ€ ๋Š˜์–ด๋‚˜๋Š” ๋งŒํผ ๋†๋„๊ฐ€ ๋น„๋ก€ํ•ด์„œ ๋‚ฎ์•„์ง€๊ธฐ ๋•Œ๋ฌธ์—, ํฌ์„๋ฐฐ์ˆ˜๋Š” ์›๋ž˜ ๋†๋„์™€ ํฌ์„๋œ ๋†๋„์˜ ๋น„์œจ๊ณผ๋„ ๊ฐ™์Šต๋‹ˆ๋‹ค. ํฌ์„๋ฐฐ์ˆ˜๊ฐ€ 10(ํ”ํžˆ 1:10์œผ๋กœ ํ‘œ๊ธฐ)์ด๋ผ๋Š” ๊ฒƒ์€ ์‹œ๋ฃŒ 1์— ๋Œ€ํ•ด ์ „์ฒด๊ฐ€ 10์ด ๋˜๋„๋ก ๋งŒ๋“ค์—ˆ๋‹ค๋Š” ๋œป์ž…๋‹ˆ๋‹ค.

๋†์ถ• ์‹œ๋ฃŒ์— ํฌ์„์•ก์„ ๋”ํ•ด ๋” ํฌ๊ณ  ๋ฌฝ์€ ์ตœ์ข… ๋ถ€ํ”ผ๋ฅผ ๋งŒ๋“  ๋ชจ์Šต
์ดˆ๊ธฐ ์‹œ๋ฃŒ์— ํฌ์„์•ก์„ ๋„ฃ์œผ๋ฉด ์ „์ฒด ๋ถ€ํ”ผ๊ฐ€ ๋Š˜๊ณ  ๋†๋„๋Š” ๋‚ฎ์•„์ง‘๋‹ˆ๋‹ค.

๊ณ„์‚ฐ๊ธฐ ์‚ฌ์šฉ ๋ฐฉ๋ฒ•

์ดˆ๊ธฐ(์‹œ๋ฃŒ) ๋ถ€ํ”ผ, ์ฆ‰ ๋†์ถ•๋œ ์›์•ก(์Šคํ†ก)์˜ ์–‘์„ ์ž…๋ ฅํ•˜๊ณ , ์šฉ๋งค๋ฅผ ์ถ”๊ฐ€ํ•œ ๋’ค์˜ ์ตœ์ข…(์ „์ฒด) ๋ถ€ํ”ผ๋ฅผ ์ž…๋ ฅํ•˜์„ธ์š”. ๋‘ ๊ฐ’์€ ๋ฐ˜๋“œ์‹œ ๊ฐ™์€ ๋‹จ์œ„(mL, ยตL, L)๋กœ ์ž…๋ ฅํ•ด์•ผ ํ•ฉ๋‹ˆ๋‹ค. ๊ณ„์‚ฐ๊ธฐ๋Š” ํฌ์„๋ฐฐ์ˆ˜์™€ ์ด์— ํ•ด๋‹นํ•˜๋Š” ํฌ์„ ๋ฐฐ์œจ, ๊ทธ๋ฆฌ๊ณ  ์ตœ์ข… ๋ถ€ํ”ผ์— ๋„๋‹ฌํ•˜๊ธฐ ์œ„ํ•ด ์ถ”๊ฐ€ํ•ด์•ผ ํ•  ํฌ์„์•ก(์šฉ๋งค)์˜ ์–‘์„ ์•Œ๋ ค์ค๋‹ˆ๋‹ค.

๊ณต์‹ ์„ค๋ช…

ํ•ต์‹ฌ ๊ณต์‹์€ $$\text{DF} = \frac{\text{V}_{\text{์ตœ์ข…}}}{\text{V}_{\text{์ดˆ๊ธฐ}}}$$ ์ž…๋‹ˆ๋‹ค. ํ•„์š”ํ•œ ์šฉ๋งค์˜ ์–‘์€ ์ตœ์ข… ๋ถ€ํ”ผ์—์„œ ์‹œ๋ฃŒ ๋ถ€ํ”ผ๋ฅผ ๋บ€ ๊ฐ’์œผ๋กœ ๊ฐ„๋‹จํžˆ ๊ตฌํ•  ์ˆ˜ ์žˆ์Šต๋‹ˆ๋‹ค. ์›ํ•˜๋Š” ํฌ์„ ์ •๋„์™€ ์›์•ก ๋†๋„๋ฅผ ์•Œ๊ณ  ์žˆ๋‹ค๋ฉด ์‹์„ ๋ณ€ํ˜•ํ•ด ํฌ์„ ํ›„ ๋†๋„๋„ ๊ตฌํ•  ์ˆ˜ ์žˆ์Šต๋‹ˆ๋‹ค: $$\text{C}_{\text{์ตœ์ข…}} = \frac{\text{C}_{\text{์ดˆ๊ธฐ}}}{\text{DF}}.$$

์ตœ์ข…/์ดˆ๊ธฐ ๋ถ€ํ”ผ๋ฅผ ์ดˆ๊ธฐ/์ตœ์ข… ๋†๋„์™€ ์—ฐ๊ฒฐํ•˜๋Š” ํฌ์„ ๋ฐฐ์ˆ˜ ๊ณต์‹
ํฌ์„ ๋ฐฐ์ˆ˜๋Š” ๋ถ€ํ”ผ ๋น„์™€ ๊ฐ™์œผ๋ฉฐ, ๋†๋„ ๋น„์˜ ์—ญ์ˆ˜์™€๋„ ๊ฐ™์Šต๋‹ˆ๋‹ค.

๊ณ„์‚ฐ ์˜ˆ์‹œ

์›์•ก 2 mL๋ฅผ ๋œ์–ด๋‚ด๊ณ  ์™„์ถฉ์•ก(๋ฒ„ํผ)์„ ๋„ฃ์–ด ์ „์ฒด ๋ถ€ํ”ผ๋ฅผ 50 mL๋กœ ๋งž์ท„๋‹ค๊ณ  ๊ฐ€์ •ํ•ด ๋ด…์‹œ๋‹ค. ํฌ์„๋ฐฐ์ˆ˜๋Š” \(50 \div 2 = \mathbf{25}\)(์ฆ‰ 1:25 ํฌ์„)์ž…๋‹ˆ๋‹ค. ์ถ”๊ฐ€ํ•œ ํฌ์„์•ก์€ \(50 - 2 = \mathbf{48}\) mL์ž…๋‹ˆ๋‹ค. ์›์•ก ๋†๋„๊ฐ€ 100 mg/mL์˜€๋‹ค๋ฉด, ํฌ์„๋œ ์šฉ์•ก์˜ ๋†๋„๋Š” \(100 \div 25 = 4\) mg/mL๊ฐ€ ๋ฉ๋‹ˆ๋‹ค.

์ž์ฃผ ๋ฌป๋Š” ์งˆ๋ฌธ

ํฌ์„๋ฐฐ์ˆ˜ 10์€ 1:10๊ณผ ๊ฐ™์€ ๊ฑด๊ฐ€์š”? ๋„ค, ๊ฐ™์Šต๋‹ˆ๋‹ค. DF๊ฐ€ 10์ด๋ผ๋Š” ๊ฒƒ์€ ์‹œ๋ฃŒ๊ฐ€ ์ตœ์ข… ๋ถ€ํ”ผ์˜ 1/10์ด๋ผ๋Š” ์˜๋ฏธ์ด๋ฉฐ, ์ด๋ฅผ 1:10์œผ๋กœ ํ‘œ๊ธฐํ•ฉ๋‹ˆ๋‹ค.

์–ด๋–ค ๋‹จ์œ„๋ฅผ ์‚ฌ์šฉํ•ด์•ผ ํ•˜๋‚˜์š”? ์ดˆ๊ธฐ ๋ถ€ํ”ผ์™€ ์ตœ์ข… ๋ถ€ํ”ผ๊ฐ€ ๊ฐ™์€ ๋‹จ์œ„์ด๊ธฐ๋งŒ ํ•˜๋ฉด ์–ด๋–ค ๋ถ€ํ”ผ ๋‹จ์œ„๋“  ์‚ฌ์šฉํ•  ์ˆ˜ ์žˆ์Šต๋‹ˆ๋‹ค. ํฌ์„๋ฐฐ์ˆ˜๋Š” ๋‹จ์œ„๊ฐ€ ์—†๋Š” ๋ฌด์ฐจ์› ๊ฐ’์ด๊ธฐ ๋•Œ๋ฌธ์ž…๋‹ˆ๋‹ค.

์—ฐ์† ํฌ์„(serial dilution)์€ ์–ด๋–ป๊ฒŒ ๊ณ„์‚ฐํ•˜๋‚˜์š”? ๊ฐ ๋‹จ๊ณ„์˜ ํฌ์„๋ฐฐ์ˆ˜๋ฅผ ๋ชจ๋‘ ๊ณฑํ•˜๋ฉด ๋ฉ๋‹ˆ๋‹ค. 1:10 ํฌ์„์„ ์„ธ ๋ฒˆ ์—ฐ์† ๊ฑฐ์น˜๋ฉด ์ „์ฒด ํฌ์„๋ฐฐ์ˆ˜๋Š” \(10 \times 10 \times 10 = 1000\)์ด ๋ฉ๋‹ˆ๋‹ค.

์ตœ์ข… ์—…๋ฐ์ดํŠธ: