MCP๋กœ ์—ฐ๊ฒฐ โ†’

๊ณ„์‚ฐ ์ž…๋ ฅ

๊ณต์‹

๊ด‘๊ณ 

๊ฒฐ๊ณผ

ํฌ์„ ๋ฐฐ์ˆ˜
10
1 : 10 (fold dilution)
์‹œ๋ฃŒ ๋ถ€ํ”ผ 1
ํฌ์„์•ก ๋ถ€ํ”ผ 9
์ด ๋ถ€ํ”ผ 10

ํฌ์„ ๋ฐฐ์ˆ˜๋ž€?

ํฌ์„ ๋ฐฐ์ˆ˜(DF, Dilution Factor)๋Š” ์‹œ๋ฃŒ๋ฅผ ๋ช‡ ๋ฐฐ๋กœ ํฌ์„ํ–ˆ๋Š”์ง€๋ฅผ ๋‚˜ํƒ€๋‚ด๋Š” ๊ฐ’์ž…๋‹ˆ๋‹ค. ์ผ์ •ํ•œ ๋ถ€ํ”ผ์˜ ์‹œ๋ฃŒ๋ฅผ ๋œ์–ด๋‚ด๊ณ  ์—ฌ๊ธฐ์— ํฌ์„์•ก(์šฉ๋งค, ์™„์ถฉ์•ก, ๋ฌผ ๋“ฑ)์„ ๋”ํ•˜๋ฉด, ํฌ์„ ๋ฐฐ์ˆ˜๋Š” ์ตœ์ข… ์ด ๋ถ€ํ”ผ๋ฅผ ์›๋ž˜ ์‹œ๋ฃŒ ๋ถ€ํ”ผ๋กœ ๋‚˜๋ˆˆ ๋น„์œจ์ด ๋ฉ๋‹ˆ๋‹ค. ์˜ˆ๋ฅผ ๋“ค์–ด DF๊ฐ€ 10์ด๋ผ๋ฉด ์‹œ๋ฃŒ๊ฐ€ 10๋ฐฐ๋กœ ํฌ์„๋˜์—ˆ๋‹ค๋Š” ๋œป์ด๋ฉฐ, ํ”ํžˆ 1:10์œผ๋กœ ํ‘œ๊ธฐํ•ฉ๋‹ˆ๋‹ค.

์‹œ๋ฃŒ ๋ถ€ํ”ผ์™€ ํฌ์„์•ก ๋ถ€ํ”ผ๊ฐ€ ํ•ฉ์ณ์ ธ ๋” ํฐ ์ด ๋ถ€ํ”ผ๋ฅผ ์ด๋ฃจ๋Š” ๋ชจ์Šต
ํฌ์„ ๋ฐฐ์ˆ˜๋Š” ์ด ๋ถ€ํ”ผ(์‹œ๋ฃŒ์™€ ํฌ์„์•ก์˜ ํ•ฉ)๋ฅผ ์›๋ž˜ ์‹œ๋ฃŒ ๋ถ€ํ”ผ์™€ ๋น„๊ตํ•œ ๊ฐ’์ž…๋‹ˆ๋‹ค.

๊ณ„์‚ฐ๊ธฐ ์‚ฌ์šฉ๋ฒ•

ํ”ผํŽซ์œผ๋กœ ๋œ์–ด๋‚ธ ์‹œ๋ฃŒ์˜ ๋ถ€ํ”ผ์™€ ์ƒˆ๋กœ ๋”ํ•œ ํฌ์„์•ก์˜ ๋ถ€ํ”ผ๋ฅผ ์ž…๋ ฅํ•˜์„ธ์š”. ๋‘ ๋ถ€ํ”ผ๋Š” ๋ฐ˜๋“œ์‹œ ๊ฐ™์€ ๋‹จ์œ„(mL, ยตL, L)๋ฅผ ์‚ฌ์šฉํ•ด์•ผ ํ•ฉ๋‹ˆ๋‹ค. ๊ณ„์‚ฐ๊ธฐ๋Š” ํฌ์„ ๋ฐฐ์ˆ˜์™€ ํ•จ๊ป˜ ํ•ฉ์ณ์ง„ ์ด ๋ถ€ํ”ผ๋ฅผ ์•Œ๋ ค ์ค๋‹ˆ๋‹ค. ๊ฒฐ๊ณผ๊ฐ€ ๋น„์œจ์ด๊ธฐ ๋•Œ๋ฌธ์— ์‹ค์ œ ๋‹จ์œ„๋Š” ์„œ๋กœ ์•ฝ๋ถ„๋˜์–ด ์‚ฌ๋ผ์ง€๋ฏ€๋กœ, ์ค‘์š”ํ•œ ๊ฒƒ์€ ๋‹จ์œ„๋ฅผ ์ผ๊ด€๋˜๊ฒŒ ๋งž์ถ”๋Š” ๊ฒƒ๋ฟ์ž…๋‹ˆ๋‹ค.

๊ณต์‹ ํ’€์ด

ํฌ์„ ๋ฐฐ์ˆ˜๋Š” ๋‹ค์Œ๊ณผ ๊ฐ™์ด ๊ณ„์‚ฐํ•ฉ๋‹ˆ๋‹ค.

$$\text{DF} = \frac{\text{V}_{sample} + \text{V}_{diluent}}{\text{V}_{sample}}$$

๋ถ„์ž๋Š” ํ˜ผํ•ฉ ํ›„์˜ ์ด ๋ถ€ํ”ผ์ด๊ณ , ๋ถ„๋ชจ๋Š” ์›๋ž˜ ์‹œ๋ฃŒ์˜ ๋ถ€ํ”ผ์ž…๋‹ˆ๋‹ค. ํฌ์„๋œ ๋†๋„๋ฅผ ๊ตฌํ•˜๋ ค๋ฉด ์›๋ž˜ ๋†๋„๋ฅผ ํฌ์„ ๋ฐฐ์ˆ˜๋กœ ๋‚˜๋ˆ„๋ฉด ๋ฉ๋‹ˆ๋‹ค.

ํฌ์„ ๋ฐฐ์ˆ˜ ๊ณต์‹์„ ์ด ๋ถ€ํ”ผ๋ฅผ ์‹œ๋ฃŒ ๋ถ€ํ”ผ๋กœ ๋‚˜๋ˆˆ ๋ถ„์ˆ˜๋กœ ํ‘œ์‹œ
ํฌ์„ ๋ฐฐ์ˆ˜๋Š” ์ด ๋ถ€ํ”ผ๋ฅผ ์‹œ๋ฃŒ ๋ถ€ํ”ผ๋กœ ๋‚˜๋ˆˆ ๊ฐ’๊ณผ ๊ฐ™์Šต๋‹ˆ๋‹ค.

๊ณ„์‚ฐ ์˜ˆ์‹œ

์„ธ๊ท  ๋ฐฐ์–‘์•ก 1 mL๋ฅผ ๋ฐฐ์ง€(broth) 9 mL์— ๋„ฃ์—ˆ๋‹ค๊ณ  ๊ฐ€์ •ํ•ด ๋ด…์‹œ๋‹ค. ์ด ๋ถ€ํ”ผ๋Š” \(1 + 9 = 10\) mL์ž…๋‹ˆ๋‹ค. ํฌ์„ ๋ฐฐ์ˆ˜๋Š” \(10 / 1 = 10\)์ด๋ฉฐ, 1:10 ํฌ์„์œผ๋กœ ํ‘œ๊ธฐํ•ฉ๋‹ˆ๋‹ค. ์›๋ž˜ ๋†๋„๊ฐ€ \(10^{8}\) cells/mL์˜€๋‹ค๋ฉด, ํฌ์„๋œ ์‹œ๋ฃŒ์˜ ๋†๋„๋Š” ์ด์ œ \(10^{7}\) cells/mL๊ฐ€ ๋ฉ๋‹ˆ๋‹ค.

์ž์ฃผ ๋ฌป๋Š” ์งˆ๋ฌธ

ํฌ์„ ๋ฐฐ์ˆ˜์™€ ํฌ์„ ๋น„์œจ์€ ๊ฐ™์€ ๊ฑด๊ฐ€์š”? ๋ฐ€์ ‘ํ•˜๊ฒŒ ๊ด€๋ จ๋˜์–ด ์žˆ์Šต๋‹ˆ๋‹ค. ํฌ์„ ๋ฐฐ์ˆ˜ 10์€ 1:10 ํฌ์„(์‹œ๋ฃŒ 1, ํฌ์„์•ก 9, ์ด 10 ๋ถ€๋ถ„)์— ํ•ด๋‹นํ•ฉ๋‹ˆ๋‹ค.

๋‹จ์œ„๊ฐ€ ๊ฒฐ๊ณผ์— ์˜ํ–ฅ์„ ์ฃผ๋‚˜์š”? ๋‘ ๋ถ€ํ”ผ๊ฐ€ ๊ฐ™์€ ๋‹จ์œ„๋ฅผ ์“ฐ๋Š” ํ•œ ์˜ํ–ฅ์„ ์ฃผ์ง€ ์•Š์Šต๋‹ˆ๋‹ค. ํฌ์„ ๋ฐฐ์ˆ˜๋Š” ๋‹จ์œ„๊ฐ€ ์—†๋Š” ๋ฌด์ฐจ์› ๊ฐ’์ž…๋‹ˆ๋‹ค.

์ตœ์ข… ๋†๋„๋Š” ์–ด๋–ป๊ฒŒ ๊ตฌํ•˜๋‚˜์š”? ์ฒ˜์Œ ๋†๋„๋ฅผ ํฌ์„ ๋ฐฐ์ˆ˜๋กœ ๋‚˜๋ˆ„๋ฉด ๋ฉ๋‹ˆ๋‹ค. \(\text{C}_{์ตœ์ข…} = \text{C}_{์ดˆ๊ธฐ} / \text{DF}\).

์ตœ์ข… ์—…๋ฐ์ดํŠธ: