MCP๋กœ ์—ฐ๊ฒฐ โ†’

๊ณ„์‚ฐ ์ž…๋ ฅ

๋‘ ๋ถ€ํ”ผ ๋ชจ๋‘ ๊ฐ™์€ ๋‹จ์œ„๋ฅผ ์‚ฌ์šฉํ•˜์„ธ์š”(์˜ˆ: mL ๋˜๋Š” ยตL). ์ตœ์ข… ๋ถ€ํ”ผ = ์‹œ๋ฃŒ + ํฌ์„์•ก.

๊ณต์‹

๊ด‘๊ณ 

๊ฒฐ๊ณผ

ํฌ์„ ๋ฐฐ์ˆ˜
10
๋ฐฐ (ร—)
์‹œ๋ฃŒ / ์Šคํ†ก ๋ถ€ํ”ผ 1
์ตœ์ข… ์ด ๋ถ€ํ”ผ 10
์ถ”๊ฐ€ํ•  ํฌ์„์•ก 9

๋ฐฐ์ˆ˜ ํฌ์„์ด๋ž€?

๋ฐฐ์ˆ˜ ํฌ์„์€ 'X๋ฐฐ ํฌ์„' ๋˜๋Š” '1:X'๋กœ ํ‘œ๊ธฐํ•˜๋ฉฐ, ์šฉ๋งค๋ฅผ ๋”ํ–ˆ์„ ๋•Œ ์šฉ์•ก์ด ๋ช‡ ๋ฐฐ๋‚˜ ๋ฌฝ์–ด์ง€๋Š”์ง€๋ฅผ ๋‚˜ํƒ€๋ƒ…๋‹ˆ๋‹ค. ์˜ˆ๋ฅผ ๋“ค์–ด 10๋ฐฐ ํฌ์„์ด๋ผ๊ณ  ํ•˜๋ฉด ์ตœ์ข… ์šฉ์•ก์˜ ๋†๋„๊ฐ€ ์›๋ž˜ ์Šคํ†ก(stock) ์šฉ์•ก์˜ 1/10์ด ๋œ๋‹ค๋Š” ๋œป์ž…๋‹ˆ๋‹ค. ๋ฐฐ์ˆ˜ ํฌ์„์€ ์ƒ๋ฌผํ•™ยทํ™”ํ•™ยท์ž„์ƒ ๊ฒ€์‚ฌ์‹ค์—์„œ ๋งค์ผ ์“ฐ๋Š” ํ‘œํ˜„์œผ๋กœ, ์—ฐ์† ํฌ์„(serial dilution), ์‹œ์•ฝ ์ค€๋น„, ๋ถ„์„(assay) ์„ธํŒ… ๋“ฑ ๊ฑฐ์˜ ๋ชจ๋“  ์ž‘์—…์ด ์ด ๋ฐฐ์ˆ˜๋ฅผ ์ •ํ™•ํžˆ ๋งž์ถ”๋Š” ๋ฐ์„œ ์‹œ์ž‘๋ฉ๋‹ˆ๋‹ค.

ํ”Œ๋ผ์Šคํฌ์—์„œ ์†Œ๋Ÿ‰์˜ ์‹œ๋ฃŒ๋ฅผ ๋” ํฐ ์ตœ์ข… ๋ถ€ํ”ผ์˜ ์•ก์ฒด์— ๋”ํ•˜๋Š” ๋ชจ์Šต์„ ๋ณด์—ฌ์ฃผ๋Š” ๋‹ค์ด์–ด๊ทธ๋žจ, ๋ฐฐ์ˆ˜ ํฌ์„์„ ์„ค๋ช…
๋ฐฐ์ˆ˜ ํฌ์„์€ ์†Œ๋Ÿ‰์˜ ์‹œ๋ฃŒ์— ํฌ์„์•ก์„ ๋”ํ•ด ๋” ํฐ ์ตœ์ข… ๋ถ€ํ”ผ๋กœ ๋งŒ๋“œ๋Š” ๊ฒƒ์ž…๋‹ˆ๋‹ค.

๊ณ„์‚ฐ๊ธฐ ์‚ฌ์šฉ ๋ฐฉ๋ฒ•

๋จผ์ € ์ถœ๋ฐœ์ ์ด ๋˜๋Š” ์‹œ๋ฃŒ(๋˜๋Š” ์Šคํ†ก) ๋ถ€ํ”ผ๋ฅผ ์ž…๋ ฅํ•˜๊ณ , ํฌ์„ ํ›„ ์›ํ•˜๋Š” ์ตœ์ข… ์ด ๋ถ€ํ”ผ๋ฅผ ์ž…๋ ฅํ•˜์„ธ์š”. ๋‘ ๊ฐ’์€ ๋ฐ˜๋“œ์‹œ ๊ฐ™์€ ๋‹จ์œ„๋ฅผ ์จ์•ผ ํ•ฉ๋‹ˆ๋‹ค. ๋ฐ€๋ฆฌ๋ฆฌํ„ฐ(mL), ๋งˆ์ดํฌ๋กœ๋ฆฌํ„ฐ(ยตL), ๋ฆฌํ„ฐ(L) ์ค‘ ๋ฌด์—‡์ด๋“  ์ƒ๊ด€์—†์ง€๋งŒ ๋‘ ๊ฐ’์˜ ๋‹จ์œ„๊ฐ€ ์ผ์น˜ํ•˜๊ธฐ๋งŒ ํ•˜๋ฉด ๋ฉ๋‹ˆ๋‹ค. ๊ณ„์‚ฐ๊ธฐ๋Š” ๋ฐฐ์ˆ˜ ํฌ์„ ๋ฐฐ์ˆ˜์™€ ํ•จ๊ป˜ ์ถ”๊ฐ€๋กœ ๋„ฃ์–ด์•ผ ํ•  ํฌ์„์•ก์˜ ์–‘(์ตœ์ข… ๋ถ€ํ”ผ์—์„œ ์‹œ๋ฃŒ ๋ถ€ํ”ผ๋ฅผ ๋บ€ ๊ฐ’)์„ ์•Œ๋ ค์ค๋‹ˆ๋‹ค.

๊ณต์‹ ํ’€์ด

ํฌ์„ ๋ฐฐ์ˆ˜๋Š” ์ตœ์ข… ๋ถ€ํ”ผ๋ฅผ ์‹œ๋ฃŒ ๋ถ€ํ”ผ๋กœ ๋‚˜๋ˆˆ ๋น„์œจ์ผ ๋ฟ์ž…๋‹ˆ๋‹ค:

$$\text{๋ฐฐ์ˆ˜} = \frac{V_{\text{์ตœ์ข…}}}{V_{\text{์‹œ๋ฃŒ}}}$$

ํฌ์„์„ ํ•ด๋„ ์šฉ์งˆ์˜ ์–‘ ์ž์ฒด๋Š” ๋ณ€ํ•˜์ง€ ์•Š๊ธฐ ๋•Œ๋ฌธ์—, ์ด ๋น„์œจ์€ ๋†๋„ ๋น„์œจ์ธ \(C_{\text{์Šคํ†ก}} / C_{\text{์ตœ์ข…}}\)๊ณผ๋„ ๊ฐ™์Šต๋‹ˆ๋‹ค. ๋”ฐ๋ผ์„œ 5๋ฐฐ ํฌ์„์„ ํ•˜๋ฉด ๋†๋„๋Š” 1/5๋กœ ๋–จ์–ด์ง‘๋‹ˆ๋‹ค. ํ•„์š”ํ•œ ํฌ์„์•ก ๋ถ€ํ”ผ๋Š” ๋‹จ์ˆœํžˆ \(V_{\text{์ตœ์ข…}} - V_{\text{์‹œ๋ฃŒ}}\)์ž…๋‹ˆ๋‹ค.

๋ฐฐ์ˆ˜๏ผ์ตœ์ข… ๋ถ€ํ”ผรท์‹œ๋ฃŒ ๋ถ€ํ”ผ๏ผ์›์•ก ๋†๋„รท์ตœ์ข… ๋†๋„๋ฅผ ๋ณด์—ฌ์ฃผ๋Š” ๊ณต์‹ ๋ถ„์„
๋ฐฐ์ˆ˜ ํฌ์„์€ ๋ถ€ํ”ผ ๋น„์œจ๊ณผ ๊ฐ™์œผ๋ฉฐ, ์ด๋Š” ๋†๋„ ๋น„์œจ๊ณผ๋„ ๊ฐ™์Šต๋‹ˆ๋‹ค.

์˜ˆ์ œ๋กœ ์‚ดํŽด๋ณด๊ธฐ

์Šคํ†ก ์šฉ์•ก 2 mL๋ฅผ ํ”ผํŽซ์œผ๋กœ ์˜ฎ๊ธด ๋’ค ์ด 50 mL๊ฐ€ ๋˜๋„๋ก ๋งž์ท„๋‹ค๊ณ  ํ•ฉ์‹œ๋‹ค. ์ด๋•Œ ๋ฐฐ์ˆ˜ ํฌ์„์€ \(50 / 2 = \textbf{25}\)๋ฐฐ(1:25 ํฌ์„)์ž…๋‹ˆ๋‹ค. ์ถ”๊ฐ€๋กœ ๋„ฃ๋Š” ํฌ์„์•ก์€ \(50 - 2 = 48\) mL์ž…๋‹ˆ๋‹ค. ๋งŒ์•ฝ ์Šคํ†ก ๋†๋„๊ฐ€ 100 mg/mL์˜€๋‹ค๋ฉด, ์ตœ์ข… ๋†๋„๋Š” \(100 / 25 = 4\) mg/mL๊ฐ€ ๋ฉ๋‹ˆ๋‹ค.

์ž์ฃผ ๋ฌป๋Š” ์งˆ๋ฌธ

'1:10 ํฌ์„'์€ 10๋ฐฐ ํฌ์„๊ณผ ๊ฐ™์€๊ฐ€์š”? ๋Œ€๋ถ€๋ถ„์˜ ์‹คํ—˜์‹ค์—์„œ๋Š” ๊ทธ๋ ‡์Šต๋‹ˆ๋‹ค. ์‹œ๋ฃŒ 1๋งŒํผ์„ ๋„ฃ์–ด ์ตœ์ข… ์ด ๋ถ€ํ”ผ 10์ด ๋˜๋„๋ก ๋งŒ๋“ค๋ฉด 10๋ฐฐ ํฌ์„์ด ๋ฉ๋‹ˆ๋‹ค. ๋‹ค๋งŒ ์ฃผ์˜ํ•˜์„ธ์š”. ์–ด๋–ค ์‚ฌ๋žŒ๋“ค์€ '1:10'์„ ์‹œ๋ฃŒ 1์— ํฌ์„์•ก 10์„ ๋”ํ•œ๋‹ค๋Š” ๋œป(์ฆ‰ 11๋ฐฐ ํฌ์„)์œผ๋กœ ์“ฐ๊ธฐ๋„ ํ•ฉ๋‹ˆ๋‹ค. ์ด ๊ณ„์‚ฐ๊ธฐ๋Š” ์ตœ์ข… ์ด ๋ถ€ํ”ผ๋ฅผ ๊ธฐ์ค€์œผ๋กœ ํ•˜๋ฏ€๋กœ, ๋ฐ˜๋“œ์‹œ ์ด ๋ถ€ํ”ผ๋ฅผ ์ž…๋ ฅํ•˜์„ธ์š”.

์ตœ์ข… ๋†๋„๋„ ๊ตฌํ•  ์ˆ˜ ์žˆ๋‚˜์š”? ๋„ค. ์Šคํ†ก ๋†๋„๋ฅผ ํ™”๋ฉด์— ํ‘œ์‹œ๋œ ๋ฐฐ์ˆ˜๋กœ ๋‚˜๋ˆ„๋ฉด ๋ฉ๋‹ˆ๋‹ค.

์–ด๋–ค ๋‹จ์œ„๋ฅผ ์จ์•ผ ํ•˜๋‚˜์š”? ๋‘ ์ž…๋ ฅ๊ฐ’์ด ๊ฐ™์€ ๋‹จ์œ„๋งŒ ์“ด๋‹ค๋ฉด ์–ด๋–ค ๋ถ€ํ”ผ ๋‹จ์œ„๋“  ๊ดœ์ฐฎ์Šต๋‹ˆ๋‹ค. ๋ฐฐ์ˆ˜ ์ž์ฒด๋Š” ๋‹จ์œ„๊ฐ€ ์—†๋Š” ๋ฌด์ฐจ์› ๊ฐ’์ด๊ธฐ ๋•Œ๋ฌธ์ž…๋‹ˆ๋‹ค.

์ตœ์ข… ์—…๋ฐ์ดํŠธ: